---

Simon Lab at the FHCRC

---

Yeast Screen of FDA-Approved Anticancer Drugs and Related Compounds

---

This information has not been endorsed by the National Cancer Institute or the Fred Hutchinson Cancer Research Center.

---

Table of Yeast Strains

Categorya	Relevant Genotypeb
Nucleotide Excision Repair	rad1c rad2 rad3-1 rad14 rad7 rad16 rad23 rad26
O6-Methylguanine Reversal	mgt1
Base Excision Repair	apn1 mag1 ung1 pol4 cdc2-2 ogg1 ntg1
Mismatch Repair	msh2 msh3 (msh6) d mlh1 pms1 (pol30-52)
Replication Fidelity	rad27 pol3-01 pol2-4
Post-Replication repair	rad6 rad18 rev1 rev2 rev3
Recombinational Repair	rad50 rad51 rad52 rad54 rad55 rad57 mre11 xrs2 rad51, rad55, rad57 (triple) mre11, xrs2 (double)
Nonhomologous End Joining	hdf1
DNA Checkpoints	rad9 rad17 rad24 mec1-1 mec2-1 mec3 pol2-12 pds1
Spindle Assembly Checkpoints	mad1 mad2 mad3 bub1 bub2 bub3
Growth Control	GAL1p::CLN2 (GAL1p::CLN3-2) GPD1p::CLN3-2 cln1, cln2 (double) clb2 sic1 RAS2 val19 ira1
Miscellaneous	sgs1 tel1 imp2 pso2 eca39 (srs2)
Drug Sensitivity	erg6 pdr1, pdr3 (double) erg6, pdr1, pdr3 (triple)

---

^a Some mutants could be assigned to more than one category. A variety of double mutants comprising different categories are not listed.
^b Strain background: A364A, MATalpha ade2 leu2 trp1ura3 cyh1.
Point mutations are as indicated. Most disruptions (designated by gene name alone) are marked with LEU2, some with TRP1, URA3 or HIS3, and some are unmarked.
The majority of the strains have a module containing two halves of the ADE2 gene flanking URA3 for measuring sister chromatid exchange.
^c Genes shown in bold have mammalian homologs.
^d Mutants listed in parentheses are still under construction.

---

Return to the Yeast Screen Main Page.
Return to the Simon Lab Home Page.

---