Welcome to the Paulovich Lab's Protein Biodosimetry Project home page.

This Project is part of the Medical Countermeasures Against Radiological and Nuclear Threats program of the Department of Health and Human Services, Office of Public Health Emergency Medical Countermeasures, and the National Institutes of Health.

Introduction: Moderate exposure to ionizing radiation can be survived with appropriate clinical intervention, which ranges from supportive care to stem cell transplant, depending on dose of exposure.  Therefore, rapid triage of exposure victims based on biodosimetry is essential for saving lives and optimizing deployment of resources during a disaster.  Current biodosimetry based on parameters such as time to onset of vomiting, lymphocyte depletion kinetics, and the appearance of dicentric chromosomes are less than ideal because they require at least 2-3 days for analysis and they are not easily automated.  The goal of this project is to develop a sensitive and automated biodosimetric assays based on rapid proteomic changes in response to radiation.

The first phase of this project was dedicated to screening for proteomic changes in response to radiation using commercially available antibodies.  The screen was done by Western Blot using commercial antibodies against protein lysates from both Lymphocytes and Lymphoblastoid Cell Lines. Below are links to the data generated during this screen.