|
Parkhurst Lab reagents available from the Development Studies Hybridoma Bank
| Antigen |
monoclonal line |
species |
refs |
|
| Rho1 |
P1D9 |
fly |
3 |
|
|
|
|
| Wash |
P3H3 |
fly |
8, 12 |
|
|
|
|
| Wash |
P4C9 |
fly |
12 |
|
|
|
|
| WASP |
P5E1 |
fly |
12 |
| WASP |
P3B1 |
fly |
12 |
|
|
|
|
| SCAR |
P1C1 |
fly |
12 |
| SCAR |
P1C8 |
fly |
12 |
|
|
|
|
| Whamy |
P4A8 |
fly |
12 |
| Whamy |
P1D1 |
fly |
12 |
|
|
|
|
| p120catenin |
P1B2 |
fly |
3, unpublished |
| p120catenin |
P4B2 |
fly |
3, unpublished |
|
|
|
|
| dSir2 |
P2E2 |
fly |
4, unpublished |
| dSir2 |
P4A10 |
fly |
4, unpublished |
Parkhurst Lab reagents available from the Bloomington Drosophila Stock Center
| Stock # |
Name |
chromosome |
refs |
• wimp (RpII140 – RNA Polymerase II subunit) |
| 5874 |
wimp, rucuca/TM3, Sb |
antimorphic allele |
1 |
• Rho1 GTPase |
| 9477 |
w*; b pr cn Rho1[1B] px sp/CyO |
null allele |
2,3,5 |
• washout (Wiskott-Aldrich Syndrome family protein) |
| 28285 |
w*; wash[Δ185]/CyO |
null allele |
6,9 |
• degringolade |
| 35525 |
w*; dgrn[DK]/TM3, Sb |
null allele |
9 |
• "Red" balancers (sChFP : sqh driven, ChFP protein) |
| 35522 |
FM7a, P{w+; sChFP} |
x |
11 |
| 35523 |
w*; Sco/CyO, P{w+, sChFP} |
2nd |
11 |
| 35524 |
w*; Gl/TM3, Sb P{w+, sChFP} |
3nd |
11 |
• actin binding domain
(sChMCA : sqh driven, ChFP protein, moesin α-helical-coiled and actin binding site) |
| 35519 |
w* P{w+, sChMCA}#12 |
X |
10 |
| 35520 |
w*; P{w+, sChMCA}#22 |
2nd |
10 |
| 35521 |
w*; P{w+, sChMCA}#31 |
3nd
| 10 |
• Rho1 GTPase (GFP fusion protein under control of endogenous Rho1 promoter) |
| 9527 |
P{Rho1.GFP}, w* |
X |
5 |
| 9528 |
w*; P{Rho1.GFP} |
2nd |
5 |
| 24762 |
w*; P{Rho1.GFP}30 |
3rd |
5 |
• actin (inducible RFP fusion protein) |
| 24777 |
P{UASp-RFP.actin}13, w* |
X |
7 |
| 24778 |
w*; P{UASp-RFP.actin}29 |
2nd |
7 |
| 24779 |
w*; P{UASp-RFP.actin}38 |
3rd |
7 |
• Cappuccino (inducible GFP fusion protein) |
| 24763 |
P{UASp-GFP.Capu}10, w* |
X |
5 |
| 24764 |
w*; P{UASp-GFP.Capu}20 |
2nd |
5 |
• Spire – C isoform (inducible GFP fusion protein) |
| 24765 |
w*; P{UASp-GFP.SpireC}21 |
2nd |
5 |
| 24766 |
w*; P{UASp-GFP.SpireC}32 |
3rd |
5 |
• Spire – D isoform (inducible GFP fusion protein) |
| 24767 |
w*; P{UASp-GFP.SpireD}30 |
3rd |
5 |
• sisyphus, unconventional myosin 10A (myosin XV homolog) |
| (inducible RFP or GFP fusion proteins) |
| 24780 |
P{UASp-Myo10A.GFP}10, w* |
X |
7 |
| 24781 |
w*; P{UASp-Myo10A.GFP}20 |
2nd |
7 |
| 24782 |
w*; P{UASp-Myo10A.GFP}30 |
3rd |
7 |
| 24783 |
P{UASp-Myo10A.mRFP}11, w* |
X |
7 |
| 24784 |
w*; P{UASp-Myo10A.mRFP}22 |
2nd |
7 |
| 24785 |
w*; P{UASp-Myo10A.mRFP}33 |
3rd |
7 |
References cited.
- Parkhurst SM and Ish-Horowicz D (1991). wimp, a dominant maternal-effect mutation, reduces transcription of a specific subset of segmentation genes in Drosophila. Genes Dev. 5, 341-357.
- Magie CR, Meyer M, Gorsuch M and Parkhurst SM (1999). Mutations in the Rho1 small GTPase disrupt morphogenesis and segmentation during early Drosophila development. Development 126, 5353-5364.
- Magie CR, Pinto-Santini D and Parkhurst SM (2002). Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction formation during Drosophila development. Development 129, 3771-3782.
- Rosenberg MI and Parkhurst SM (2002). Drosophila Sir2 is required for heterochromatic silencing and function of euchromatic Hairy/E(spl) family bHLH repressors in segmentation and sex determination. Cell 109, 447-458.
- Rosales-Nieves AE, Johndrow JE, Keller LC, Magie CR, Pinto-Santini D and Parkhurst SM (2006). Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire, and Cappuccino. Nature Cell Biol. 8, 367-376.
- Linardopoulou EV, Parghi SS, Friedman C, Osborn GE, Parkhurst SM and Trask BJ. (2007). Human subtelomeric WASH genes encode a new subclass of the WASP family. PLoS Genetics 3, e237.
- Liu R, Woolner S, Johndrow JE, Metzger D, Flores A and Parkhurst SM (2008). Sisyphus, the Drosophila myosin XV homolog, traffics within filopodia transporting key sensory and adhesion cargos. Development 135, 53-63.
- Liu R, Abreu-Blanco MT, Barry KC, Linardopoulou EV, Osborn GE and Parkhurst SM (2009). Wash functions downstream of Rho and links linear and branched action nucleation factors. Development 136, 2849-2860.
- Barry KC, Abed M, Kenyagin D, Werwie TR, Boico O, Orian A and Parkhurst SM (2011). The Drosophila STUbL protein Degringolade limits HES function during embryogenesis. Development 138, 1759-1769.
- Abreu-Blanco MT, Verboon JM and Parkhurst SM (2011). Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. J. Cell Biol. 193, 455-464.
- Abreu-Blanco MT, Verboon JM, Liu R, Watts JJ and Parkhurst SM. (2012). Submitted.
- Rodriguez-Mesa E, Abreu-Blanco MT, Rosales-Nieves AE and Parkhurst SM (2012). Developmental expression of Drosophila Wiskott Aldrich Syndrome family proteins. Dev. Dyn. 241, 608-626
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Reagents and Protocols
