Reagents and Protocols

Parkhurst Lab reagents available from the Bloomington Drosophila Stock Center

Stock # Name

chromosome

refs


•  wimp (RpII140 – RNA Polymerase II subunit)

5874

 wimp, rucuca/TM3, Sb

antimorphic allele

1


•  Rho1 GTPase

9477

 w*; b pr cn Rho1[1B] px sp/CyO

null allele

2,3,5


•  washout  (Wiskott-Aldrich Syndrome family protein)

28285

 w*; wash[Δ185]/CyO

null allele

6,9

 
• Rho1 GTPase (GFP fusion protein under control of endogenous Rho1 promoter)

9527

 P{Rho1.GFP}, w*

X

5

9528

 w*; P{Rho1.GFP}       

2nd

5

24762

 w*; P{Rho1.GFP}30

3rd

5

 
• actin  (inducible RFP fusion protein)

24777

 P{UASp-RFP.actin}13, w*

X

7

24778

 w*; P{UASp-RFP.actin}29

2nd

7

24779

 w*; P{UASp-RFP.actin}38

3rd

7


• Cappuccino   (inducible GFP fusion protein)

24763

 P{UASp-GFP.Capu}10, w*

X

5

24764

 w*; P{UASp-GFP.Capu}20

2nd

5


•  Spire – C isoform   (inducible GFP fusion protein)

24765

 w*; P{UASp-GFP.SpireC}21

2nd

5

24766

 w*; P{UASp-GFP.SpireC}32

3rd

5


• Spire – D isoform   (inducible GFP fusion protein)

24767

 w*; P{UASp-GFP.SpireD}30

3rd

5


• sisyphus, unconventional myosin 10A (myosin XV homolog)

   (inducible RFP or GFP fusion proteins)

24780

 P{UASp-Myo10A.GFP}10, w*

X

7

24781

 w*; P{UASp-Myo10A.GFP}20

2nd

7

24782

w*; P{UASp-Myo10A.GFP}30        

3rd

7

24783

 P{UASp-Myo10A.mRFP}11, w* 

X

7

24784

 w*; P{UASp-Myo10A.mRFP}22     

2nd

7

24785

 w*; P{UASp-Myo10A.mRFP}33 

3rd

7

 

Parkhurst Lab reagents available from the Development Studies Hybridoma Bank 

Antigen                          monoclonal line                   species         refs
Rho1 P1D9 fly 3
Wash P3H3 fly 8
p120catenin P1B2 fly 3, unpublished
p120catenin P4B2 fly 3, unpublished
dSir2 P2E2 fly 4, unpublished
dSir2 P4A10 fly 4, unpublished

 References cited.

  1. Parkhurst SM and Ish-Horowicz D (1991). wimp, a dominant maternal-effect mutation, reduces transcription of a specific subset of segmentation genes in Drosophila.  Genes Dev. 5, 341-357.
  2. Magie CR, Meyer M, Gorsuch M and Parkhurst SM (1999). Mutations in the Rho1 small GTPase disrupt morphogenesis and segmentation during early Drosophila development.  Development 126, 5353-5364.
  3. Magie CR, Pinto-Santini D and Parkhurst SM (2002). Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction formation during Drosophila development.  Development 129, 3771-3782.
  4. Rosenberg MI and Parkhurst SM (2002). Drosophila Sir2 is required for heterochromatic silencing and function of euchromatic Hairy/E(spl) family bHLH repressors in segmentation and sex determination.  Cell 109, 447-458.
  5. Rosales-Nieves AE, Johndrow JE, Keller LC, Magie CR, Pinto-Santini D and Parkhurst SM (2006). Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire, and Cappuccino.  Nature Cell Biol. 8, 367-376.
  6. Linardopoulou EV, Parghi SS, Friedman C, Osborn GE, Parkhurst SM and Trask BJ. (2007). Human subtelomeric WASH genes encode a new subclass of the WASP family. PLoS Genetics 3, e237.
  7. Liu R, Woolner S, Johndrow JE, Metzger D, Flores A and Parkhurst SM (2008). Sisyphus, the Drosophila myosin XV homolog, traffics within filopodia transporting key sensory and adhesion cargos. Development 135, 53-63.
  8. Liu R, Abreu-Blanco MT, Barry KC, Linardopoulou EV, Osborn GE and Parkhurst SM (2009). Wash functions downstream of Rho and links linear and branched action nucleation factors.  Development 136, 2849-2860.

 

Protocols

construction_fly

Last updated 9/23/2009
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