Protocols

Zebrafish Protocols

• RNA in situ protocolPDF
• Mailing live embryos PDF
• Whole Mount Antibody Staining HTML
• Zebrafish Sperm Cryopreservation PDF
• DNA Injection Protocol PDF
• Bleaching embryos HTML
• Aaron?s ET BAC Recombination PDF
• Totally Anal RNA in situ Probe Synthesis HTML
• Determining ENU Concentration HTML
• RMO44 Antibody Staining HTML | PDF
• HRP detection with DAP HTML
• val genotyping with dCAPS HTML | PDF
• lzr genotyping with dCAPS HTML | PDF
• Lab Recipes HTML | PDF
• Lab probes list HTML | PDF
• Antibody staining protocol PDF
• Instructions for making bridged coverslips PDF

Video Protocols

• A high-throughput method for zebrafish sperm cryopreservation and in vitro fertilization.
• Making gynogenetic diploid zebrafish by early pressure.
• Generating chimeric zebrafish embryos by transplantation.

Plasmid Databases

• University of Washington Labs plasmid databases
• Moens Lab plasmid database

Click HERE for Tilling Protocols