Shipment of AAV Vector Plasmids

AAV vector plasmids are shipped in 10 mM Tris-Cl pH 7.5, about 1 microgram per sample. Cut the samples apart if necessary, add water and use to transform bacteria. These plasmids should be grown in bacteria with low propensity to cause rearrangements by recombination or repair pathways, such as SURE bacteria (Stratagene) or JC8111 (Boissy and Astell, Gene 35:179, 1985). The terminal repeats of AAV can rearrange even in these bacteria, so it is prudent to use restriction enzymes that cut in the TRs to check for the lack of rearrangement. AhdI cuts once in each TR and is useful in this regard. Other TR cutters are MscI, BssHII, XmaI, and BglI.