Acrylamide Urea Gel (35 ml)

  10% 15%
40/2% acrylamide 10 ml 13.1 ml
10X TBE 3.5 ml 3.5 ml
Urea 15 g 15 g
H20 10ml 7.0 ml

 

Microwave ~10 seconds and stir until dissolved

Degas 1-2 min

Add 0.3 ml 10% ammonium persulfate

Add 35 microliters TEMED.

Pour Gel and wait at least 20 minutes before removing comb.

The gel can be stored overnight at room temp. if the top of the gel is wet with water and is wrapped tightly in Saran Wrap


Steve Hahn

Last modified10/16/98

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