Method for spotting yeast cells for cell growth assay

Ted Young, 2009

Fill wells of 96 well microplate with 240 microliters 10% glycerol. 

Add 60 microliters culture.  Make five fold serial dilutions by removing 60 microliters of first dilution and  pipeting into 240 microliters and so on.

Insert yeast multi-pin apparatus into 96 well plate.   Gently pull out being careful not to touch sides of the plate.  Quickly put on Petri plate. 

This will give uniform plating of cells.