Stark's Buffer

M.Fero — 1/17/2001

Final Concentration

5x SSC

25 mM NaPO4, pH6.5

5x Denhardt's Solution

0.25 mg/mL Torula RNA

50% (v/v) Formamide

Stock Solutions

20x SSC: 3 M NaCl, 0.3 M NaCitrate, pH7.0

50x Denhardt's: 1% (w/v) BSA, 1% (w/v) PVP-40, 1% (w/v) Ficoll-400

10 mg/mL Torula RNA (boil to dissolve)

1 M NaPO4, pH 6.5

Preparation

To make 1 L. of Stark's Buffer mix:

250 mL 20x SSC

25 mL 1M NaPO4, pH6.5

100 mL 50x Denhardt's solution

25 mL 10 mg/mL Torula RNA (boil just prior to adding)

122.5 mL H2O

500 mL Formamide