Fero Lab Protocols

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DNA
ABC Random Amplification
Alcohol Precipitation of DNA
AML sample DNA preparation
Electroelution
Fluorometry
New Oligonucleotides
Southern Hybridization
Stark's Prehybridization Buffer
Tail DNA prep
Q-PCR
Nanodrop

RNA
Amino-allyl labeling
BioAnalyzer
TRIzol plus RNEasy Mini
RNEasy Midi
TRIzol product insert
RNA amplification
RNA gel electrophoresis
Protein
Histone Kinase Assay
Western blot
p27 ELISA

Bacteria
General Cloning Procedures
Preparing Electrocompentent cells
Electroporation & Blue/White Selection
Colony Hybridization
Large scale plasmid prep, PEG method
Large scale plasmid prep, Qiagen/CsCl
Ligation Optimization
Plasmid Miniprep

Flow Cytometry
Lineage analysis of blood
MEF nuclei prep for DNA content
Flow of surface and nuclear markers
BrdU and PI Staining Cells for Flow
Simultaneous BrdU and surface staining
Flow sorting fibroblasts for GFP
Setup and Use of the Cytometer
96-well Flow Setup Record (PDF)
Counting Fluorescently Stained Cells

Cell culture
Culturing MEFs
Calcium Phosphate Coprecipitation
Harvesting murine bone marrow
Purification of L-KS+ marrow cells
Hematopoietic CFC assays
Retrovirus Production
STO and ES Cell Culture
XC assay of MoMLV

Mice
Complete Blood Cell Counts from Mice
Genotyping p27 knockout mice
Tail DNA prep
Toe ID System
Using the MPD
Necropsy Form
GE µCT scans
Xenogen Imager

Histology
Immunoflourescence of MEFs
Mouse Necropsy
Necropsy form
Paraffin embedding and Sectioning
Staining Protocols
H & E staining
BrdU Staining Histological Sections
X-gal Staining
HRP-DAB Immunostaining
AP-BCIP/NBT Immunostaining
GFP & BrdU In Situ Immunoflourescence
Fluorescent In Situ Hybridization

Product Manuals
TRIzol reagent
BioRad Bradford protein assay
Qiagen RNEasy Mini
Sorvall HB6 Rotor Specs
ImageJ User Guide
DirectPCR Tail DNA Preps

Lab Maintenance
Solutions
Useful Formulae
Formulae
Power Curves
Simple dilutions


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