Fero Lab Protocols

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DNA ABC Random Amplification Alcohol Precipitation of DNA AML sample DNA preparation Electroelution Fluorometry New Oligonucleotides Southern Hybridization Stark's Prehybridization Buffer Tail DNA prep Q-PCR Nanodrop	RNA Amino-allyl labeling BioAnalyzer TRIzol plus RNEasy Mini RNEasy Midi TRIzol product insert RNA amplification RNA gel electrophoresis	Protein Histone Kinase Assay Western blot p27 ELISA
Bacteria General Cloning Procedures Preparing Electrocompentent cells Electroporation & Blue/White Selection Colony Hybridization Large scale plasmid prep, PEG method Large scale plasmid prep, Qiagen/CsCl Ligation Optimization Plasmid Miniprep	Flow Cytometry Lineage analysis of blood MEF nuclei prep for DNA content Flow of surface and nuclear markers BrdU and PI Staining Cells for Flow Simultaneous BrdU and surface staining Flow sorting fibroblasts for GFP Setup and Use of the Cytometer 96-well Flow Setup Record (PDF) Counting Fluorescently Stained Cells	Cell culture Culturing MEFs Calcium Phosphate Coprecipitation Harvesting murine bone marrow Purification of L-KS+ marrow cells Hematopoietic CFC assays Retrovirus Production STO and ES Cell Culture XC assay of MoMLV
Mice Complete Blood Cell Counts from Mice Genotyping p27 knockout mice Tail DNA prep Toe ID System Using the MPD Necropsy Form GE µCT scans Xenogen Imager	Histology Immunoflourescence of MEFs Mouse Necropsy Necropsy form Paraffin embedding and Sectioning Staining Protocols H & E staining BrdU Staining Histological Sections X-gal Staining HRP-DAB Immunostaining AP-BCIP/NBT Immunostaining GFP & BrdU In Situ Immunoflourescence Fluorescent In Situ Hybridization	Product Manuals TRIzol reagent BioRad Bradford protein assay Qiagen RNEasy Mini Sorvall HB6 Rotor Specs ImageJ User Guide DirectPCR Tail DNA Preps
Lab Maintenance Solutions	Useful Formulae Formulae Power Curves Simple dilutions

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