Hematoxylin and Eosin (H&E) Staining:
Wei-Ming Chien & Emily CaufieldSee also Matthew's protocol
- Deparafinization:
- Xylene (x2 or 3 changes)
- 100% EtOH (x2)
- 95% EtOH (x1)
- 70% EtOH (x1)
- 50% EtOH (x1)
- ddH2O (x2)
- Procedure:
- (Note: Unless otherwise specified, slides should be dipped 10 times in each solution)
- Hematoxylin, 2 minutes (x1)
- ddH20 (x2)
- Ammonia solution 10 dips (x1)
- ddH2O (x2)
- 50% EtOH (x1)
- 70% EtOH (x1)
- 95% EtOH (x1)
- 100% EtOH (x1)
- 100% MeOH (x1)
- Eosin 3-5 dips
- 100% MeOH (x1)
- 100% EtOH (x2)
- Xylene
Mount slides with permount
- Reagents:
- Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450
- Eosin: 1x stock. Cat #837.
- Ammonia Solution: 0.084 (w/v) NH4OH (1 L H2O 3 mL 28% w/v NH4OH stock).
